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Leptin, Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2057
- Product Name Leptin, Human, ELISA assay
- Product Description The human Leptin Kit is a sandwich ELISA. The capture antibody is a monoclonal human Leptin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Leptin concentration are added to these wells and allowed to complex with the bound Leptin antibody. A biotinylated anti-Leptin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Leptin present in the samples. The purpose of this kit is the in-vitro quantitative detection of human leptin in culture supernatant, serum, plasma (Heparin) and plasma (EDTA) only when used as directed. Not recommended for tissue lysate detection of leptin.
- Alternative Names Leptin; Obesity factor; Lep; Ob; Obese protein;
- Application(s) ELISA
- Specificity Human Leptin Mouse and rat leptin sequences show >80% amino acid identity with human leptin but direct cross reactivity to rodent or non-human species leptin's with this kit has yet to be tested.
- Species Reactivity Human
- Immunogen Description The standard used in this kit is recombinant human Leptin, amino acids 22-167 produced in and purified from E. coli.
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Leptin Kit is a sandwich ELISA. The capture antibody is a monoclonal human Leptin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Leptin concentration are added to these wells and allowed to complex with the bound Leptin antibody. A biotinylated anti-Leptin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Leptin present in the samples. The purpose of this kit is the in-vitro quantitative detection of human leptin in culture supernatant, serum, plasma (Heparin) and plasma (EDTA) only when used as directed. Not recommended for tissue lysate detection of leptin.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Leptin in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Leptin
- Specificity Human Leptin Mouse and rat leptin sequences show >80% amino acid identity with human leptin but direct cross reactivity to rodent or non-human species leptin's with this kit has yet to be tested.
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard used in this kit is recombinant human Leptin, amino acids 22-167 produced in and purified from E. coli.
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Leptin; Obesity factor; Lep; Ob; Obese protein;
- Uniprot Number P41159
- Uniprot Number/Name P41159 (LEP_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
