Luteinzing hormone subunit beta (LH-B), Guinea pig Polyclonal Antibody

Product Info

Product Name Luteinzing hormone subunit beta (LH-B), Guinea pig Polyclonal Antibody
Product Description google Guinea Pig anti-Luteinzing hormone subunit beta (LH-B) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Alternative Names Lutropin subunit beta [Precursor]; Luteinizing hormone subunit beta; LSH-beta; LSH-B; LH-B; Lutropin beta chain; Interstitial cell-stimulating hormone
Application(s) IHC-Frozen
Antibody Host Guinea Pig
Antibody Type Polyclonal
Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested
Species Reactivity Sheep
Immunogen Description A synthetic peptide corresponding to the antigenic region within the ovine LH residue.
Conjugate Unconjugated
Purity Description Neat serum.
Regulatory Status For research use only.

Specifications

Product Description Guinea Pig anti-Luteinzing hormone subunit beta (LH-B) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s) IHC-Frozen
Application Details IHC. A concentration of 1 in 3000 is recommended for IHC. IHC performed in sheep pituitary demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of LH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Luteinzing hormone subunit beta (LH-B)
Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested
Target Host Species Sheep
Species Reactivity Sheep
Antibody Host Guinea Pig
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide corresponding to the antigenic region within the ovine LH residue.
Purity Description Neat serum.
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Lutropin subunit beta [Precursor]; Luteinizing hormone subunit beta; LSH-beta; LSH-B; LH-B; Lutropin beta chain; Interstitial cell-stimulating hormone
Uniprot Number P01231
Uniprot Number/Name P01231 (LSHB_SHEEP)
Scientific Background Luteinizing hormone promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. It exists as a heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin, and gonatropin.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

The cryostat section of the sheep pituitary was incubated in guinea pig polyclonal antibodies to ovine luteinizing hormone at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals in the sheep brain are intensely stained (brown). This figure shows staining of cells when no pre-absorption is performed.

Citations & References

Specific References Sanchez NS, Quinn KE, Ashley AK, Ashley RL (2017). In the ovine pituitary, CXCR4 is localized in gonadotropes and somatotropes and increases with elevated serum progesterone. Domest Anim Endocrinol. In press.
General References Clarke IJ, Tobin VA, Pompolo S, Pereira A (2005). Effects of changing Gonadotropin-releasing hormone pulse frequency and estrogen treatment on levels of estradiol receptor -alpha and induction of Fos and phosphorylated cyclic adenosine monophosphate response element binding protein in pituitary gonadotropes: studies in hypothalamo-pituitary disconnected ewes. Endocrinology 146 (3) pp. 1128-1137.