Application DetailsIHC. A concentration of 1 in 3000 is recommended for IHC. IHC performed in sheep pituitary demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of LH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetLuteinzing hormone subunit beta (LH-B)
SpecificitySpecificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested
Target Host SpeciesSheep
Antibody HostGuinea Pig
Immunogen DescriptionA synthetic peptide corresponding to the antigenic region within the ovine LH residue.
Purity DescriptionNeat serum.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsIt is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundLuteinizing hormone promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. It exists as a heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin, and gonatropin.
The cryostat section of the sheep pituitary was incubated in guinea pig polyclonal antibodies to ovine luteinizing hormone at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals in the sheep brain are intensely stained (brown). This figure shows staining of cells when no pre-absorption is performed.
Specific ReferencesSanchez NS, Quinn KE, Ashley AK, Ashley RL (2017). In the ovine pituitary, CXCR4 is localized in gonadotropes and somatotropes and increases with elevated serum progesterone. Domest Anim Endocrinol. In press.
General ReferencesClarke IJ, Tobin VA, Pompolo S, Pereira A (2005). Effects of changing Gonadotropin-releasing hormone pulse frequency and estrogen treatment on levels of estradiol receptor -alpha and induction of Fos and phosphorylated cyclic adenosine monophosphate response element binding protein in pituitary gonadotropes: studies in hypothalamo-pituitary disconnected ewes. Endocrinology 146 (3) pp. 1128-1137.