Major intrinsic protein (MIP-2), Mouse, ELISA assay

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Catalog Number
BEK-2179

    Product Info

  • Product Name Major intrinsic protein (MIP-2), Mouse, ELISA assay
  • Product Description google The mouse MIP-2 Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse MIP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known MIP-2 concentration are added to these wells and allowed to complex with the bound MIP-2 antibody. A biotinylated mouse monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MIP-2 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names MP26; Major intrinsic protein; MIP; Aquaporin-0; MIP-26;
  • Application(s) ELISA
  • Specificity Mouse MIP-2
  • Species Reactivity Mouse
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A28-N100
  • Range 15.6 pg/mL - 1,000 pg/mL
  • Sensitivity < 5 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The mouse MIP-2 Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse MIP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known MIP-2 concentration are added to these wells and allowed to complex with the bound MIP-2 antibody. A biotinylated mouse monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MIP-2 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Major intrinsic protein (MIP-2) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Major intrinsic protein (MIP-2)
  • Specificity Mouse MIP-2
  • Target Host Species Mouse
  • Species Reactivity Mouse
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A28-N100
  • Range 15.6 pg/mL - 1,000 pg/mL
  • Sensitivity < 5 pg/mL
  • Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names MP26; Major intrinsic protein; MIP; Aquaporin-0; MIP-26;
  • Uniprot Number P10889
  • Uniprot Number/Name P10889 (CXCL2_MOUSE)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References

  • Specific References Kanaya K et al. (2014) Innate immune responses and neuroepithelial degeneration and regeneration in the mouse olfactory mucosa induced by intranasal administration of Poly(I:C). Cell Tissue Res. 2014 Jul;357(1):279-99.