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Matrix metalloproteinase-2 (MMP-2), Human, ELISA assay

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Catalog Number
BEK-2067
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    Product Info

  • Product Name Matrix metalloproteinase-2 (MMP-2), Human, ELISA assay
  • Product Description google The human Matrix metalloproteinase-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-2 concentration are added to these wells and allowed to complex with the bound MMP-2 antibody. A biotinylated human MMP-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-2 in samples such as culture supernatant, serum and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names 72 kDa type IV collagenase; EC 3.4.24.24; 72 kDa gelatinase; Matrix metalloproteinase-2; MMP-2; Gelatinase A; TBE-1; MMP2; CLG4A;
  • Application(s) ELISA
  • Specificity Human MMP-2. This kit also shows reactivity with mouse MMP-2.
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A30-C660
  • Range 625 pg/mL - 4,0000 pg/mL
  • Sensitivity < 10 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The human Matrix metalloproteinase-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-2 concentration are added to these wells and allowed to complex with the bound MMP-2 antibody. A biotinylated human MMP-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-2 in samples such as culture supernatant, serum and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Matrix metalloproteinase-2 (MMP-2) in Culture Supernatant, Serum, Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Matrix metalloproteinase-2 (MMP-2)
  • Specificity Human MMP-2. This kit also shows reactivity with mouse MMP-2.
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A30-C660
  • Range 625 pg/mL - 4,0000 pg/mL
  • Sensitivity < 10 pg/mL
  • Sample Type Culture Supernatant, Plasma (Heparin), Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names 72 kDa type IV collagenase; EC 3.4.24.24; 72 kDa gelatinase; Matrix metalloproteinase-2; MMP-2; Gelatinase A; TBE-1; MMP2; CLG4A;
  • Uniprot Number P08253
  • Uniprot Number/Name P08253 (MMP2_HUMAN)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.