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Matrix metalloproteinase-2 (MMP-2), Rat, ELISA assay
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US$547.00
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Catalog Number
BEK-2187
- Product Name Matrix metalloproteinase-2 (MMP-2), Rat, ELISA assay
- Product Description The rat Matrix metalloproteinase-2 (MMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal rat MMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known MMP-2 concentration are added to these wells and allowed to complex with the bound MMP-2 antibody. A biotinylated anti-rat MMP-2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat MMP-2 in samples such as culture supernatant, serum and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names 72 kDa type IV collagenase; 72 kDa gelatinase; Matrix metalloproteinase-2; MMP-2; Gelatinase A; TBE-1; MMP2; CLG4A;
- Application(s) ELISA
- Specificity Natural and recombinant Rat Mmp 2; assay will likely detect both proform and active forms of MMP2 and cannot distingish between the two.
- Species Reactivity Rat
- Immunogen Description The standard and immunogen for this assay is recombinant rat MMP-2 protein expressed in and purified from a mammalian cell expression system. The immunogen sequence cooresponds to amino acids 30-662 of rat MMP-2 protein.
- Range 625 pg/ml-4,0000 pg/ml
- Sensitivity <10 pg/ml
- Regulatory Status For research use only.
Product Info
- Product Description The rat Matrix metalloproteinase-2 (MMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal rat MMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known MMP-2 concentration are added to these wells and allowed to complex with the bound MMP-2 antibody. A biotinylated anti-rat MMP-2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat MMP-2 in samples such as culture supernatant, serum and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Matrix metalloproteinase-2 (MMP-2) in Culture Supernatant, Serum, Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Matrix metalloproteinase-2 (MMP-2)
- Specificity Natural and recombinant Rat Mmp 2; assay will likely detect both proform and active forms of MMP2 and cannot distingish between the two.
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard and immunogen for this assay is recombinant rat MMP-2 protein expressed in and purified from a mammalian cell expression system. The immunogen sequence cooresponds to amino acids 30-662 of rat MMP-2 protein.
- Range 625 pg/ml-4,0000 pg/ml
- Sensitivity <10 pg/ml
- Sample Type Culture Supernatant, Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names 72 kDa type IV collagenase; 72 kDa gelatinase; Matrix metalloproteinase-2; MMP-2; Gelatinase A; TBE-1; MMP2; CLG4A;
- Uniprot Number P33436
- Uniprot Number/Name P33436 (MMP2_RAT)
- Scientific Background The matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases. All MMPs are synthesized as inactive proenzymes. The activation of these proenzymes is a critical step that leads to degradation of extracellular matrix components such as fibronectin and collagen type III. MMP2 is activated by other MMPs such as MMP14 and MMP16. MMP2 is known to degrade collagen types IV, V, VII and X as well as gelatin type I.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
