Matrix metalloproteinase-9 (MMP-9), Human, ELISA assay

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Catalog Number
BEK-2073

    Product Info

  • Product Name Matrix metalloproteinase-9 (MMP-9), Human, ELISA assay
  • Product Description google The human Matrix metalloproteinase-9 (MMP-9) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MMP-9 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-9 concentration are added to these wells and allowed to complex with the bound MMP-9 antibody. A biotinylated human MMP-9 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-9 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-9 in samples such as culture supernatant, serum, plasma (Heparin), saliva and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names MMP-9; MMP9; Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; CLG4B;
  • Application(s) ELISA
  • Specificity Detects human pro and active MMP-9 and MMP-9 bound to TIMP. Human; does not react with rat or mouse MMP-9
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A20-D707
  • Range 156 pg/mL - 10,000 pg/mL
  • Sensitivity < 5 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The human Matrix metalloproteinase-9 (MMP-9) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MMP-9 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-9 concentration are added to these wells and allowed to complex with the bound MMP-9 antibody. A biotinylated human MMP-9 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-9 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-9 in samples such as culture supernatant, serum, plasma (Heparin), saliva and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Matrix metalloproteinase-9 (MMP-9) in Culture Supernatant, Serum, Plasma (Heparin), Saliva, Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Matrix metalloproteinase-9 (MMP-9)
  • Specificity Detects human pro and active MMP-9 and MMP-9 bound to TIMP. Human; does not react with rat or mouse MMP-9
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A20-D707
  • Range 156 pg/mL - 10,000 pg/mL
  • Sensitivity < 5 pg/mL
  • Sample Type Culture Supernatant, Plasma (Heparin), Saliva, Serum, Urine
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names MMP-9; MMP9; Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; CLG4B;
  • Uniprot Number P14780
  • Uniprot Number/Name P14780 (MMP9_HUMAN)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References

  • Specific References Shang L et al. (2022) Effects of the triple therapy of carnosine glycoside, edaravone, and Xueshuantong in hemorrhagic cerebral infarction. Am J Transl Res. 14(2):1024-1033 Application: Human serum.