Melanocyte-stimulating hormone, alpha (Alpha-MSH), Guinea pig Polyclonal Antibody

Product Info

Product Name Melanocyte-stimulating hormone, alpha (Alpha-MSH), Guinea pig Polyclonal Antibody
Product Description google Guinea Pig anti-Melanocyte-stimulating hormone, alpha (Alpha-MSH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Alternative Names Proopiomelanocortin; POMC; alpha-melanocyte stimulating hormone; melanotropin alpha
Application(s) IHC-Frozen
Antibody Host Guinea Pig
Antibody Type Polyclonal
Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with human, rat and sheep. Other species have not yet been tested.
Species Reactivity Human, Rat, Sheep
Immunogen Description A synthetic peptide (Ac-SYSMEHFRWGKPV) corresponding to the amino acids 138-150 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to a carrier protein to enhance the immunological response.
Conjugate Unconjugated
Purity Description Neat serum
Regulatory Status For research use only.

Specifications

Product Description Guinea Pig anti-Melanocyte-stimulating hormone, alpha (Alpha-MSH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s) IHC-Frozen
Application Details IHC. A concentration of 1 in 2,000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of alpha MSH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Melanocyte-stimulating hormone, alpha (Alpha-MSH)
Specificity Specificity was demonstrated by immunohistochemistry. This antibody is known to react with human, rat and sheep. Other species have not yet been tested.
Target Host Species Human
Species Reactivity Human, Rat, Sheep
Antibody Host Guinea Pig
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide (Ac-SYSMEHFRWGKPV) corresponding to the amino acids 138-150 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to a carrier protein to enhance the immunological response.
Purity Description Neat serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Proopiomelanocortin; POMC; alpha-melanocyte stimulating hormone; melanotropin alpha
Uniprot Number P01189
Uniprot Number/Name P01189 (COLI_HUMAN)
Scientific Background Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates. MSH increases the pigmentation of skin by increasing the melanin production in melanocytes. MSH is produced in the pituitary gland and belongs to the POMC family.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Figure shows staining of cells in sheep hypothalamus using Guinea pig polyclonal antibody to human alpha MSH (GP-030-50) at 1:1000 followed by incubation with FITC labeled goat anti-guinea pig secondary antibodies.
Immunohistochemical detection of alpha MSH-immunoreactive neurons in the human infundibular nucleus using the gp-030-50 primary antiserum (1:40,000), followed by its visualization with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Immunohistochemical identification of alpha MSH-immunoreactive neurons in cryostat sections from the hypothalamic arcuate nucleus of the rat. The signal was detected with the gp-030-50 primary antiserum (1:40,000), followed by its visualization with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Citations & References

General References Cardoso RC, Alves BR, Sharpton SM, Williams GL, Amstalden M (2015). Nutritional Programming of Accelerated Puberty in Heifers: Involvement of Pro-Opiomelanocortin Neurones in the Arcuate Nucleus. J Neuroendocrinol. 2015 Aug;27(8):647-57.

Grayson BE, Allen SE, Billes SK, Williams SM, Smith MS, Grove KL (2006). Prenatal development of hypothalamic neuropeptide systems in the nonhuman primate. Neuroscience. 2006 Dec 28;143(4):975-86.