Alternative NamesGamma-melanocyte stimulating hormone; Melanotropin gamma; Corticotropin-lipotropin [Precursor]; Pro-opiomelanocortin; POMC
Application(s)IHC-Frozen
Antibody HostGuinea Pig
Antibody TypePolyclonal
SpecificityHuman. Specificity was demonstrated by immunohistochemistry. This antiserum is known to react with sheep gamma MSH. Other species have not yet been tested.
Species ReactivityHuman, Sheep
Immunogen DescriptionA synthetic peptide (YVMGHFRWDRFG) corresponding to the amino acids 77-88 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to carrier protein to enhance the immunological response.
Application DetailsIHC. A concentration of 1 in 2000 is recommended for IHC. This antibody shows excellent staining on sheep and human tissue. Rodent tissues demonstrate high background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHuman. Specificity was demonstrated by immunohistochemistry. This antiserum is known to react with sheep gamma MSH. Other species have not yet been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Sheep
Antibody HostGuinea Pig
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (YVMGHFRWDRFG) corresponding to the amino acids 77-88 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to carrier protein to enhance the immunological response.
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsIt is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGamma-melanocyte stimulating hormone; Melanotropin gamma; Corticotropin-lipotropin [Precursor]; Pro-opiomelanocortin; POMC
Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates.
The cryostat section of the sheep hypothalamus was incubated in guinea pig polyclonal antibodies to the gamma melanocyte-stimulating hormone at the dilution of 1:1000 overnight followed by incubation with FITC- conjugated secondary antibodies. Cell bodies and nerve terminals in the sheep brain are intensely stained.
Immunohistochemical detection of gamma MSH-immunoreactive neurons in the human infundibular nucleus using the GP-031-50 primary antiserum (1:40,000) and the biotinylated secondary antibody-ABC method with nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.