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Metalloproteinase inhibitor 3 (TIMP-3), Human, ELISA assay
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Catalog Number
BEK-2099
- Product Name Metalloproteinase inhibitor 3 (TIMP-3), Human, ELISA assay
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Product Description
The human Metalloproteinase inhibitor 3 (TIMP-3) Kit is a sandwich ELISA. The capture antibody is a monoclonal human TIMP-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TIMP-3 concentration are added to these wells and allowed to complex with the bound TIMP-3 antibody. A biotinylated human TIMP-3 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TIMP-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TIMP-3 in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and saliva. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
The matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases that degrade extracellular matrix components such as fibronectin and collagen type III. The tissue inhibitors of metalloproteinase (TIMPs) complex with these matrix metalloproteinases and irreversibly inactivate them. The TIMP proteins are homologous and are either secreted in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). Metalloproteinase inhibitor 3 (TIMP3) is known to inhibit MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. - Alternative Names Tissue inhibitor of metalloproteinases 3; TIMP-3; MIG-5; TIMP3;
- Application(s) ELISA
- Specificity Human TIMP-3
- Species Reactivity Human
- Immunogen Description The standard and antigen is recombinant TIMP-3 amino acids 24-211 produced in and purified from mammalian cell line tissue culture.
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 2 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
The human Metalloproteinase inhibitor 3 (TIMP-3) Kit is a sandwich ELISA. The capture antibody is a monoclonal human TIMP-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TIMP-3 concentration are added to these wells and allowed to complex with the bound TIMP-3 antibody. A biotinylated human TIMP-3 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TIMP-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TIMP-3 in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and saliva. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
The matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases that degrade extracellular matrix components such as fibronectin and collagen type III. The tissue inhibitors of metalloproteinase (TIMPs) complex with these matrix metalloproteinases and irreversibly inactivate them. The TIMP proteins are homologous and are either secreted in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). Metalloproteinase inhibitor 3 (TIMP3) is known to inhibit MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. - Application(s) ELISA
- Application Details ELISA. For the quantification of Metalloproteinase inhibitor 3 (TIMP-3) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Saliva. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Metalloproteinase inhibitor 3 (TIMP-3)
- Specificity Human TIMP-3
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and antigen is recombinant TIMP-3 amino acids 24-211 produced in and purified from mammalian cell line tissue culture.
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 2 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Saliva, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Tissue inhibitor of metalloproteinases 3; TIMP-3; MIG-5; TIMP3;
- Uniprot Number P35625
- Uniprot Number/Name P35625 (TIMP3_HUMAN
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
- Specific References Casagrande V et al. (2021) Restoration of renal TIMP3 levels via genetics and pharmacological approach prevents experimental diabetic nephropathy. Clin Transl Med. Application: Human, serum.