MHC class I polypeptide-related sequence A (MIC-A), Human, ELISA assay

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Catalog Number
BEK-2125

    Product Info

  • Product Name MHC class I polypeptide-related sequence A (MIC-A), Human, ELISA assay
  • Product Description google
    The human MHC Class I polypeptide-related sequence A (MIC-A) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MIC-A antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIC-A concentration are added to these wells and allowed to complex with the bound MIC-A antibody. A biotinylated human MICA polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIC-A present in the samples. The purpose of this kit is the in-vitro quantitative detection of human MIC-A in culture supernatant, cell lysates and serum, only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names MIC-A; MICA;
  • Application(s) ELISA
  • Specificity Human MHC Class I polypeptide-related sequence A
  • Species Reactivity Human
  • Immunogen Description Antigen and Standard is recombinant human MIC-A amino acids 24-307 produced in and purified from a mammalian cell expression system.
  • Range 62.5 pg/mL - 4,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    The human MHC Class I polypeptide-related sequence A (MIC-A) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MIC-A antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIC-A concentration are added to these wells and allowed to complex with the bound MIC-A antibody. A biotinylated human MICA polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIC-A present in the samples. The purpose of this kit is the in-vitro quantitative detection of human MIC-A in culture supernatant, cell lysates and serum, only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of MHC class I polypeptide-related sequence A (MIC-A) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target MHC class I polypeptide-related sequence A (MIC-A)
  • Specificity Human MHC Class I polypeptide-related sequence A
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description Antigen and Standard is recombinant human MIC-A amino acids 24-307 produced in and purified from a mammalian cell expression system.
  • Range 62.5 pg/mL - 4,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Sample Type Cell Lysates, Culture Supernatant, Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names MIC-A; MICA;
  • Uniprot Number Q29983
  • Uniprot Number/Name Q29983 (MICA_HUMAN)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References