Application DetailsWestern Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). Suggested dilutions are: 1:10,00-1:20,000 (WB), 1:5,000-1:10,000 (ICC, IHC). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMicrotubule-associated protein 2 (MAP2)
SpecificityThe specificity of this antibody has been confirmed by IC. Hu, Rat, Ms, Bov
Target Host SpeciesBovine
Species ReactivityBovine, Human, Mouse, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionBovine MAP2 isolated from brain by the GTP microtubule cycling method.
Purity DescriptionIgY
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesMicrotubule-associated protein 2; MAP-2; MAP2;
Scientific BackgroundMicrotubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (red). The perikarya and dendrites of neurons are strongly and specifically stained with this antibody while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.
View of a thin section of adult rat cerebellum stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (green), Rabbit polyclonal to GFAP R-1374-50 (red) and DNA (blue). The image shows the molecular layer (outside of lobe) and granular layer; blue since its full of small neurons and the white matter in the middle.
Left: Analysis of MAP2 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken anti-MAP2 (1:10,000, red), co-stained with a mouse antibody to MAP-tau (green). Blue: DAPI nuclear stain. The MAP2 antibody stains dendrites and perikarya of neurons, while the MAP-tau antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Right: Western blot analysis of whole brain tissue lysates using chicken antibody to MAP2 (1:50,000). [1] protein standard, [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. A strong band at around 280 kDa corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at lower dilutions.
Left: Analysis of MAP2 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken anti-MAP2 (1:10,000, red), co-stained with a mouse antibody to MAP-tau (green). Blue: DAPI nuclear stain. The MAP2 antibody stains dendrites and perikarya of neurons, while the MAP-tau antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Right: Western blot analysis of whole brain tissue lysates using chicken antibody to MAP2 (1:50,000). [1] protein standard, [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. A strong band at around 280 kDa corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at lower dilutions.
Specific ReferencesMichurina A (2021) "Loss of Setd1b methyltransferase in the murine forebrain as a novel model for human intellectual disability." PhD Thesis. Application: IHC (IF) Species: Mouse.
Iwata M et al. (2019) "Regulatory mechanisms for the axonal localization of tau protein in neurons." Mol Biol Cell. 30(19):2441-57. Application: ICC/IF Species: Mouse, rat.
Duda JK et al. (2019) "The role of DLG-MAGUKs in mediating signaling specificity at the postsynaptic density." PhD Thesis. [Epub ahead of print]. Application: ICC/IF Species: Mouse.
Awashti A et al. (2018) "Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting." Science. 2018; [Epub ahead of print]. Application: IHC/ICC/IF Species: Mouse.
Wolfes AC et al. (2016) "A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes." J Gen Physiol. 2016; [Epub ahead of print]. Application: IF Species: Rat.
Dziennis S et al. (2007) "Role of signal transducer and activator of transcription-3 in estradiol-mediated neuroprotection." J Neurosci. 2007; 27(27):7268-74. Application: IHC Species: Rat.