View of a thin section of adult rat cerebellum stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (green), Rabbit polyclonal to GFAP R-1374-50 (red) and DNA (blue). The image shows the molecular layer (outside of lobe) and granular layer; blue since its full of small neurons and the white matter in the middle.
Left: Analysis of MAP2 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken anti-MAP2 (1:10,000, red), co-stained with a mouse antibody to MAP-tau (green). Blue: DAPI nuclear stain. The MAP2 antibody stains dendrites and perikarya of neurons, while the MAP-tau antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Right: Western blot analysis of whole brain tissue lysates using chicken antibody to MAP2 (1:50,000). [1] protein standard, [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. A strong band at around 280 kDa corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at lower dilutions.
Left: Analysis of MAP2 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken anti-MAP2 (1:10,000, red), co-stained with a mouse antibody to MAP-tau (green). Blue: DAPI nuclear stain. The MAP2 antibody stains dendrites and perikarya of neurons, while the MAP-tau antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Right: Western blot analysis of whole brain tissue lysates using chicken antibody to MAP2 (1:50,000). [1] protein standard, [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. A strong band at around 280 kDa corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at lower dilutions.