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LipoFluor-MR™ Ready-to-Dilute™, Mitochondria Tracing Reagent
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Catalog Number
TR-602
- Product Name LipoFluor-MR™ Ready-to-Dilute™, Mitochondria Tracing Reagent
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Product Description
LipoFluor-MR™ is a cell-permeant stain for mitochondria. This tracing reagent can be used on live cells, frozen and fixed tissue samples and is compatible with other dyes. LipoFluor-MR™ has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity.
- Application(s) IF, ICC, Live Cell IF
- Specificity Fluorescent imaging of mitochondria. Cell penetration and localisation of LipoFluor-MR™ has been confirmed in prostate cells (PNT2 and 22RV1), cardiomyocytes (H9c2) and cancer cell lines (HeLa). Mitochondrial localisation has been shown in live tissues, including live adipose tissue (sheep) and muscle tissue (sheep cardiac and skeletal) and frozen or PFA fixed muscle tissue (sheep skeletal).
- Species Reactivity Human, Mouse, Other Mammals, Rat
- Concentration 20mM
- Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.
- Regulatory Status For research use only.
Product Info
-
Product Description
LipoFluor-MR™ is a cell-permeant stain for mitochondria. This tracing reagent can be used on live cells, frozen and fixed tissue samples and is compatible with other dyes. LipoFluor-MR™ has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity.
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LipoFluor-ER2™ Ready-to-Dilute™, Endoplasmic Reticulum – Blue Tracing Reagent
- Application(s) IF, ICC, Live Cell IF
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Application Details
Fluorescent imaging of mitochondria.
Sample types: live cells, tisue (frozen and 4% paraformaldehyde). Quenching endogenous fluorescence can assist in imaging, refer to the product manual. Not suitable for application on fixed cells.
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: NO
Raman spectroscopy: NO
Ex/Em: 405 nm / 600 nm.
The typical working concentration is 20µM (1:1000 dilution) in an appropriate buffer or cell culture medium. Biosensis recommends that the end user determine optimal dilutions/concentrations. - Target Mitochondria
- Specificity Fluorescent imaging of mitochondria. Cell penetration and localisation of LipoFluor-MR™ has been confirmed in prostate cells (PNT2 and 22RV1), cardiomyocytes (H9c2) and cancer cell lines (HeLa). Mitochondrial localisation has been shown in live tissues, including live adipose tissue (sheep) and muscle tissue (sheep cardiac and skeletal) and frozen or PFA fixed muscle tissue (sheep skeletal).
- Target Host Species Human
- Species Reactivity Human, Mouse, Other Mammals, Rat
- Ex/Em Max Ex/Em: 405 nm / 600 nm
- Detection Method Fluorescence
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Kit Components
Materials provided:
LipoFluor™ Stain Reagent, 1000X, 4 vials - Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.
- Format The reagents in the LipoFluor™ RTD™ (1000X) are all supplied in a liquid format and are ready-to-dilute.
- Concentration 20mM
- Reconstitution Instructions Dilute solutions as directed in the protocol instructions.
- Storage Instructions The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date If protected from light, the liquid 20 mM stock solution vials are stable for 12 months from date of receipt, at 2-8ºC. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 60103920
- Regulatory Status For research use only.
