Application DetailsWestern blotting (1:5,000) and Immunohistochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityBovine, reacts with Pig. Other species not yet tested. Antibody detects arrestin-1 protein only and does not cross-react with the other 3 arrestin molecules.
Target Host SpeciesBovine
Species ReactivityBovine, Pig
Immunogen DescriptionRecombinant bovine arrestin-1 with the first 20 amino acids of the C-terminus truncated
Purity DescriptionAffinity purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Names48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin
Scientific BackgroundBinds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (PubMed: 8003967, PubMed: 25205354). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells (By similarity). Ref: uniprot.org
Left: Immunohistochemical analysis of arrestin-1 distribution in pig retina. Arrestin (green) is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL) and can be used to identify components of rod photoreceptor cells (cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The RNA binding protein FOX2 appears red and immunoreactivity is seen in nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA appears blue. Right: Western blot analysis of arrestin in bovine retinal extracts. The antibody stains one specific band at 48 kDa, corresponding to retinal arrestin.