Product DescriptionMouse anti-Alpha-(1,3)-fucosyltransferase 4 (CD15) Monoclonal Antibody (Unconjugated), suitable for ICC, IHC-Paraffin-embedded, FC.
Application(s)FC, ICC, IHC-Paraffin-embedded
Application DetailsImmunohistochemistry (0.5-1.0 µg/mL): tested in formalin-fixed, paraffin-embedded sections of human Hodgkin's lymphoma. Immunofluorescence ( (0.5-1.0 µg/mL) on 4% PFA fixed or acetone fixed sections. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Positive control : U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAlpha-(1,3)-fucosyltransferase 4 (CD15)
SpecificityCD15; This antibody reacts with a 220 kDa protein, CD15 / FUT4 expressed on Reed-Sternberg cells.
Target Host SpeciesHuman
Antibody IsotypeIgM, kappa
Immunogen DescriptionPurified human neutrophils were used as the immunogen.
Purity DescriptionPurified from tissue culture supernatant by PEG precipitation.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [https://www.ncbi.nlm.nih.gov/gene/2526]
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Reed Sternberg cells (also known as lacunar histiocytes for certain types) are different giant cells found with light microscopy in biopsies from individuals with Hodgkin's lymphoma (a.k.a. Hodgkin's disease, a type of lymphoma). They are usually derived from B lymphocytes, classically considered crippled germinal center B cells, meaning they have not undergone hypermutation to express their antibody. Seen against a sea of B cells, they give the tissue a moth-eaten appearance.
Classification of Hodgkin's is based on the reactive cell mixture. Immunomarkers are used (e.g., CD15 and CD30).
Reed Sternberg cells are large (30-50 microns) and are either multinucleated or have a bilobed nucleus with prominent eosinophilic inclusion-like nucleoli (thus resembling an "owl's eye" appearance). Reed Sternberg cells are CD30 and CD15 positive, usually negative for CD20 and CD45. The presence of these cells is necessary in the diagnosis of Hodgkin's lymphoma the absence of Reed Sternberg cells has very high negative predictive value. They can also be found in reactive lymphadenopathy (such as infectious mononucleosis immunoblasts which are RS like in appearance, carbamazepine associated lymphadenopathy) and very rarely in other types of non-Hodgkin lymphomas. Anaplastic large cell lymphoma may show RS-like cells as well.