Left: Rat brain cerebellum stained with mouse anti-calbindin (green, 1:1,000) and rabbit anti-calretinin (R-1800-50, red, 1:5,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast, the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer. Right: Western blot analysis of calbindin expression (antibody dilution: 1:2,000) in rat cerebellum (2), pig hippocampus (3), and cow cerebellum (4). The band at ~25 kDa corresponds to calbindin protein, heavily expressed in the cerebellum, but less in hippocampus.