Left: Rat cerebellum stained with mouse anti-CNP (green, 1:500) and chicken anti-NF-M (C-1393-50, red, 1:1,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The CNP antibody specifically stains oligodendrocytes, cells that create the myelin sheath around axons. The NF-M antibody labels axons of neuronal cells. Right: Western blot analysis of rat brain (2), rat spinal cord (3), mouse brain (4), mouse spinal cord (5). Antibody dilution: 1:2,000. Double bands at 46 and 48 kDa correspond to the major isotypes of CNP.