Product DescriptiongoogleMouse anti-Parvalbumin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative NamesParvalbumin alpha
Application(s)IHC-Frozen, WB
Antibody HostMouse
Antibody TypeMonoclonal
SpecificityHuman,reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionFull-length recombinant human protein
Product DescriptionMouse anti-Parvalbumin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s)IHC-Frozen, WB
Application DetailsWestern blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Note that this antibody does not recognize parvalbumin in rat or mouse brain homogenates on western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetParvalbumin
SpecificityHuman,reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG1
Clone Name3C9
ConjugateUnconjugated
Immunogen DescriptionFull-length recombinant human protein
Purity DescriptionAffinity purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Left: Staining of parvalbumin in adult rat cerebellum floating section by Immunohistochemistry. Mouse antibody to parvalbumin (1:1,000, green) stains dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue: Nuclear DNA stain. Right: Western blot analysis of parvalbumin expression in rat skeletal muscle homogenate (Lane 1). The antibody (1:1,000) detects parvalbumin at ~12 kDa in homogenate (Lane 1) and His-tagged parvalbumin protein (Lane 2) at slightly higher molecular weight. The antibody does not cross-react with His-tagged proteins calretinin (Lane 3) and calbindin (Lane 4). Note that this antibody does not recognize parvalbumin in rat or mouse brain lysates on western blots.
Rat cerebellum section stained with mouse anti-parvalbumin (green, 1:1,000) and chicken anti-calbindin (C-1798-50, red, 1:2,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone, and so appear green.
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