Application DetailsWestern blot (WB) at a suggested concentration of 0.5-2.0 µg/mL, Immunofluorescence 1-10 µg/mL and indirect ELISA 0.1-0.2 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Scientific BackgroundMANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Western Blot testing of anti-MANF monoclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85 cell line.