Product DescriptionMouse anti-14-3-3 protein eta Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Application(s)ICC, IHC-Frozen, WB
Application DetailsWB, ICC, IHC. Suggested dilution of 1:500-1:1,000 for IHC and ICC. Suggested dilution of 1:1,000-1:5,000 for WB. A suitable control tissue is rat spinal cord or peripheral nerve homogenate.
Target14-3-3 protein eta
SpecificityHuman 14-3-3 ETA protein
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name3G12
ConjugateUnconjugated
Immunogen DescriptionFull length recombinant 14.3.3 protein ETA expressed in and purified from E. coli.
Purity DescriptionIgG
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Names14.3.3 ; Protein AS1; YWHAH; YWHA1; tyrosine 3-monooxygenase; tryptophan 5-monooxygenase activation protein 1;
Scientific Background14.3.3 protein eta or 14.3.3 binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (Ref SwissProt). 14.3.3 protein eta is widely expressed as both homodimers and heterodimers and are concentrated in the nervous system. High concentrations of 14.3.3 protein eta have been linked to Creutzfeld Jacob Disease, Parkinson's Disease and early-onset schizopherenia.
Image shows rat mixed neuron/glial cultures stained with Mouse monoclonal antibody to 14.3.3 protein eta M-1572-100 antibody (red). Neuronal perikarya are very rich in 14.3.3 protein eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain.
Left: Analysis of 14.3.3 eta expression in HeLa cells by Immuncytochemistry using mouse antibody to 14.3.3 protein eta (red, 1:1,000). Blue: DAPI nuclear stain. The antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region. Right: Western blot analysis of whole brain lysates (lanes 2,3), and cell lysates (lanes 4-8), using mouse antibody to 14.3.3 eta (green, 1:5,000). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells. The strong band at 28 kDa corresponds to 14.3.3 eta protein.
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