Application DetailsImmunohistochemistry (IHC-Frozen). A concentration of 1.0 µg/mL is recommended to detect CD3 in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetT-cell surface glycoprotein CD3 delta chain
SpecificityThe specificity of this antibody has been confirmed by immunohistochemistry against the antigen. Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Immunogen DescriptionHuman thymocytes followed by Sezary T cells
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesT-cell surface glycoprotein CD3; CD3E; CD3G; CD3H; CD3Q; CD3Z; T cell receptor T3;
Scientific BackgroundThe T-cell receptor-CD3 complex (CD3-TCR) plays an important role in mediating signaling transduction. The CD3-TCR complex consists of either a TCR alpha/beta or TCR gamma/delta heterodimer co-expressed at the cell surface with the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These subunits are single-pass type I membrane proteins expressed on normal peripheral blood lymphocytes, thymocytes and Purkinje cells in the cerebellum.