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Actin, aortic smooth muscle, Mouse Monoclonal Antibody
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Catalog Number
M-996
- Product Name Actin, aortic smooth muscle, Mouse Monoclonal Antibody
- Product Description Mouse anti-Actin, aortic smooth muscle Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names ACTA2; ACTSA; ACTVS;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Species Reactivity Human, Mouse, Rat
- Immunogen Description A synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin. This antibody reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins.
- Conjugate Unconjugated
- Purity Description IgG
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Actin, aortic smooth muscle Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human smooth muscle actin has a predicted length of 377 residues and a MW of 42 kDa. A concentration of 0.1-0.5 µg/mL is recommended to detect alpha smooth muscle actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Actin, aortic smooth muscle
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name 1A4
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin. This antibody reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins.
- Purity Description IgG
- Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names ACTA2; ACTSA; ACTVS;
- Uniprot Number P62736
- Uniprot Number/Name P62736 (ACTA_HUMAN)
- Scientific Background Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
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Specific References
Pogribny IP et al (2013) Strain-dependent dysregulation of one-carbon metabolism in male mice is associated with choline- and folate-deficient diet-induced liver injury. FASEB J. 2013 Jun;27(6):2233-43.
Sebastiani G et al (2011) Accelerated CCl4-induced liver fibrosis in Hjv-/- mice, associated with an oxidative burst and precocious profibrogenic gene expression. PLoS One. 2011;6(9):e25138.