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Actin, cytoplasmic 1, Mouse Monoclonal Antibody
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Catalog Number
M-1613
- Product Name Actin, cytoplasmic 1, Mouse Monoclonal Antibody
- Product Description Mouse anti-Actin, cytoplasmic 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names ACTB; AX; actin; Actx;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity Beta Actin protein
- Species Reactivity Human, Mouse, Rat
- Immunogen Description A modified synthetic peptide (C-DDDIAALVIDNGSGK) corresponding to the N-terminus of beta Actin conjugated to KLH.
- Conjugate Unconjugated
- Purity Description IgG
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Actin, cytoplasmic 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.5 µg/mL is recommended for WB. Human beta actin has a predicted length of 375 residues and the MW of the monomer is 42 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect beta actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Actin, cytoplasmic 1
- Specificity Beta Actin protein
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name AC-15
- Conjugate Unconjugated
- Immunogen Description A modified synthetic peptide (C-DDDIAALVIDNGSGK) corresponding to the N-terminus of beta Actin conjugated to KLH.
- Purity Description IgG
- Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names ACTB; AX; actin; Actx;
- Uniprot Number P60709
- Uniprot Number/Name P60709 (ACTB_HUMAN)
- Scientific Background Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Beta actins coexist with gamma actins in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
