TAR DNA-binding protein 43 (TDP-43), Mouse Monoclonal Antibody

Product Info

Product Name TAR DNA-binding protein 43 (TDP-43), Mouse Monoclonal Antibody
Product Description google Mouse anti-TAR DNA-binding protein 43 (TDP-43) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Alternative Names TAR DNA-binding protein 43; TDP-43; TARDBP; TDP43;
Application(s) ICC, IHC-Frozen, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~43 kDa TDP43 protein on crude extract of mouse brain nuclear fraction. Human and Rodent. Predicted to react with other mammalian tissue.
Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
Immunogen Description This antibody was raised against recombinant full length human his-tagged TDP43 which was expressed in E. coli and purified by nickel affinity.
Conjugate Unconjugated
Purity Description IgG
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-TAR DNA-binding protein 43 (TDP-43) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Application(s) ICC, IHC-Frozen, WB
Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:5,000 is recommended for WB and IHC. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target TAR DNA-binding protein 43 (TDP-43)
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~43 kDa TDP43 protein on crude extract of mouse brain nuclear fraction. Human and Rodent. Predicted to react with other mammalian tissue.
Target Host Species Human
Species Reactivity Human, Mouse, Other Mammals (Predicted), Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG
Clone Name 3H8
Conjugate Unconjugated
Immunogen Description This antibody was raised against recombinant full length human his-tagged TDP43 which was expressed in E. coli and purified by nickel affinity.
Purity Description IgG
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names TAR DNA-binding protein 43; TDP-43; TARDBP; TDP43;
Uniprot Number Q13148
Uniprot Number/Name Q13148 (TADBP_HUMAN)
Scientific Background TAR DNA-binding protein 43 (TDP43) is a DNA and RNA-binding protein which regulates transcription and splicing (ref: SWISSPROT).
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Mouse monoclonal antibody to TAR DNA-binding protein 43 [3H8] M-1403-100 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken polyclonal antibody to GFAP C-1373-50 (green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TAR DNA-binding protein 43 antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TAR DNA-binding protein 43 and blue due to their content of DNA, stained blue with DAPI.
Left: Analysis of TDP43 expression in rat hippocampus section by Immunohistochemistry. Section was stained with mouse anti-TDP43 antibody (red, 1:2,000), and co-stained with chicken antibody to GFAP (C-1373-50, green, 1:5,000). Blue: DAPI nuclear stain. IHC method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells. Right: Western blot analysis of whole brain lysates and nuclear extracts for TDP43 expression (green, 1:2,000). [1] protein standard, [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. A strong band at 43 kDa corresponds to TDP43 protein.

TDP43 staining in fixed and permeabilized human umbilical vein endothelial cells (HUVEC) by Immunocytochemistry. TDP43-IR (green) is localized to cell nuclei as expected. Blue: Hoechst nuclear stain (left only). Red: Phalloidin staining. Primary TDP43 antibody concentration: 2 µg/mL; Magnification: 32X. Image courtesy of QBM Cell Science.

TDP43 staining in fixed and permeabilized human umbilical vein endothelial cells (HUVEC) by Immunocytochemistry. TDP43-IR (green) is localized to cell nuclei as expected. Red: Staining of von Willebrandt factor. Primary TDP43 antibody concentration: 2 µg/mL; Magnification: 32X. Image courtesy of QBM Cell Science.

Staining of fixed and permeabilized rat hippocampal cells for TDP43 (green) and NeuN (red) by Immunocytochemistry. Green: Mouse antibody to TDP43, M-1403-100 (2 µg/mL); Red: Rabbit antibody to NeuN, R-3770-100 (1:500 dilution); Blue: Hoechst nuclear dye. TDP43-IR and NeuN-IR is localized to cell nuclei as expected. Magnification: 32X. Image courtesy of QBM Cell Science.

Citations & References