Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human N-Cadherin has a predicted length of 906 residues and MW of 100 kDa. A concentration of 2.0-4.0 µg/mL is recommended for IHC to detect the protein in paraffin embedded tissues. Antigen retrieval by heat is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit; chicken; snake;
Target Host SpeciesChicken
Species ReactivityChicken, Human, Mouse, Rabbit, Rat, Snake
Immunogen DescriptionA synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
FormatLyophilized. Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt -20°C for one year. After reconstitution, at 2-8°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCDH; N-cadherin; Neural Cadherin; Cadherin-2; P-cadherin; R-cadherin; B-cadherin; E-cadherin;
Scientific BackgroundCadherins are transmembrane glycoproteins that mediate calcium ion-dependent cell adhesion. The cadherin family includes N-, P-, R-, B- and E-cadherins which are found in adherens junctions.
Specific ReferencesKawai T and Shimada M (2020). Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. Sci Rep. 10(1):6841; Application: IHC/IF, WB Species: Mouse