Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Bovine Calbindin-D has a predicted length of 261 residues and a MW of 28 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect Calbindin-D in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCalbindin-D is a highly conserved 28 kDa protein that belongs to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D has 4 active calcium binding domains. It plays a role in the regulation of calcium and calcium related signalling in neurons and during embryo development. This antibody binds only to calbindin-D-28K and not with other family members such as calretinin and calbindin-D-9K.
Western blot of mouse monoclonal antibody to Calbindin-D (M-976-100) using rat brain lysate (lanes 1 and 2), rat kidney lysate (lane 3) and rat lung tissue lysate (lane 4) at a concentration of 0.5 µg/mL. Secondary antibody HRP conjugated goat anti-mouse IgG at 1:3,000-10,000.