Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.50 µg/mL is recommended for WB. Bovine Calcineurin alpha has a predicted length of 521 aa and MW of 59 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Calcineurin alpha in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat;
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCalcineurin is a major calmodulin binding protein in the brain. It is a holoenzyme composed of a calmodulin binding subunit, a catalytic alpha subunit (calcineurin A) and a calcium binding beta subunit (calcineurin B). The calcineurin A subunit has at least two isoforms in bovine brain; alpha 1 (61 kDa) and alpha 2 (59 kDa). This antibody reacts with the isoforms of the calcineurin A subunit.