Product DescriptionMouse anti-Calmodulin Monoclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB). A concentration of 0.5 µg/mL is recommended for WB. Rat Calmodulin has a predicted length of 149 residues and a MW of 17 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB. Chicken; rat
Target Host SpeciesDictyostelium discoideum
Species ReactivityChicken, Dictyostelium discoideum, Rat
Immunogen DescriptionPurified Calmodulin from Dictyostelium discoideum conjugated to KLH
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCaM; CALM; CALM1; CALM2; CALM3; calA; camA
Scientific BackgroundCalmodulin is a small highly conserved protein with four functional calcium binding sites. Calmodulin undergoes conformational changes when it binds to calcium which can increase its affinity for target proteins. It acts directly, through interaction with key target enzymes, and indirectly, via specific kinases.