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Collagen alpha-1(III) chain, Mouse Monoclonal Antibody
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Catalog Number
M-1221
- Product Name Collagen alpha-1(III) chain, Mouse Monoclonal Antibody
- Product Description Mouse anti-Collagen alpha-1(III) chain Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Collagen alpha-1(III) chain; COL3A1;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Species Reactivity Human, Mouse, Rat
- Immunogen Description Human Collagen Type III
- Conjugate Unconjugated
- Purity Description IgG
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Collagen alpha-1(III) chain Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.50 µg/mL is recommended for WB. Human Collagen III (isoform 1) has a predicted length of 1,466 residues and a MW of 139 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Collagen alpha-1(III) chain
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name Col-29
- Conjugate Unconjugated
- Immunogen Description Human Collagen Type III
- Purity Description IgG
- Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Collagen alpha-1(III) chain; COL3A1;
- Uniprot Number P02461
- Uniprot Number/Name P02461 (CO3A1_HUMAN)
- Scientific Background Type III Collagen is a a fibrillar collagen found in most soft connective tissues such as skin, lung and intestine. At least 2 isoforms are produced by alternative splicing.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.