Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25 μg/mL is recommended for WB. Human CDK7 has a predicted length of 346 residues and MW of 39 kDa. A concentration of 0.5 µg/mL is recommended to detect the protein in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCyclin-dependent kinase 7 (CDK7)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Immunogen DescriptionRecombinant human Cyclin-dependent kinase 7 (CDK7)
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCyclin-dependent kinase 7 (CDK7) belongs to the cyclin-dependent protein kinase family. CDK7 associates with cyclin H and MAT1 to form the trimeric CDK-activating kinase (CAK) complex. CAK activates cyclin-associated kinases such as CDK4 and CDK6 by threonine phosphorylation.