Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human CDK2NA (isoform 1, p16INK4A) has a predicted length of 156 residues and MW of 17 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCyclin-dependent kinase inhibitor 2A
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Immunogen DescriptionRecombinant human Cyclin-dependent kinase inhibitor 2A
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe Cyclin-dependent kinase inhibitor 2A (CDK2NA) gene encodes several transcripts which differ from each other in their first exons. In spite of the structural and functional differences of these transcripts, they are all involved in cell cycle G1 control. Isoform 1, also known as p16INK4A, acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6 kinases. This antibody reacts specifically with this isoform (p16INK4A).