Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Cytokeratin 7 has a predicted length of 469 amino acids and MW of 51 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCytokeratin 7 (CK-7)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Immunogen DescriptionCytoskeletal preparation of the RT4 human bladder carcinoma cell line
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesKeratin; type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; KRT7; SCL; CK7; K2C7; MGC3625; MGC129731;