Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human Cytokeratin 18 has a predicted length of 430 amino acids and MW of 48 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. A concentration of 0.5-2.0 µg/mL is recommended to detect the protein in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCytokeratin 18 (CK-18)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Immunogen DescriptionHuman epidermal carcinoma A431 and MCF7 human breast cancer cell lines
Scientific BackgroundCytokeratin 18 is an acidic type I keratin and a member of intermediate filament family of proteins. Cytokeratin 18 typically forms a heterotetramer with Cytokeratin 8 to form an intermediate filament in simple single-layered epithelial cells. Cytokeratin 18 is expressed primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas.