Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human Cytokeratin 1 has a predicted length of 644 amino acids and MW of 66 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Immunogen DescriptionCytokeratin from human epidermis.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCytokeratins belong to the intermediate filament family of proteins and are classified according to their type. Type I Cytokeratins are low molecular weight and acidic whereas Type II Cytokeratins are higher weight and basic to neutral. This antibody specifically detects type II Cytokeratins 1, 5, 6, and 8. Expression of these Cytokeratins is frequently tissue specific.