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Extracellular signal-regulated kinase 1 and Extracellular signal-regulated kinase 12 (ERK-1/ERK-2), Mouse Monoclonal Antibody
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Catalog Number
M-983
- Product Name Extracellular signal-regulated kinase 1 and Extracellular signal-regulated kinase 12 (ERK-1/ERK-2), Mouse Monoclonal Antibody
- Product Description Mouse anti-Extracellular signal-regulated kinase 1 and Extracellular signal-regulated kinase 12 (ERK-1/ERK-2) Monoclonal Antibody (Unconjugated), suitable for WB.
- Alternative Names MAPK; mitogen activated protein kinase; ERK1; ERK2; Extracellular signal-regulated kinase 1; 2; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; ERK-2; MAP kinase 2; MAPK 2; p42-MAPK; ERT1;
- Application(s) WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat; yeast
- Species Reactivity Human, Mouse, Rat, Yeast
- Immunogen Description Synthetic peptide of 11 residues (HTGFLTEYVAT) conjugated to KLH. This antibody reacts with the epitope containing phosphorylated threonine and tyrosine residues within the regulatory site of active ERK1 and ERK2 (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2).
- Conjugate Unconjugated
- Purity Description IgG
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Extracellular signal-regulated kinase 1 and Extracellular signal-regulated kinase 12 (ERK-1/ERK-2) Monoclonal Antibody (Unconjugated), suitable for WB.
- Application(s) WB
- Application Details Western Blotting (WB). A concentration of 0.25-0.50 µg/mL is recommended for WB. Human ERK1 has a predicted length of 379 residues and MW of 43 kDa. Human ERK2 has a predicted length of 360 residues and MW of 41 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Extracellular signal-regulated kinase 1 and Extracellular signal-regulated kinase 12 (ERK-1/ERK-2)
- Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat; yeast
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat, Yeast
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name IL-13
- Conjugate Unconjugated
- Immunogen Description Synthetic peptide of 11 residues (HTGFLTEYVAT) conjugated to KLH. This antibody reacts with the epitope containing phosphorylated threonine and tyrosine residues within the regulatory site of active ERK1 and ERK2 (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2).
- Purity Description IgG
- Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names MAPK; mitogen activated protein kinase; ERK1; ERK2; Extracellular signal-regulated kinase 1; 2; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; ERK-2; MAP kinase 2; MAPK 2; p42-MAPK; ERT1;
- Uniprot Number P27361; P28482
-
Uniprot Number/Name
P27361 (MK03_HUMAN)
P28482 (MK01_HUMAN) - Scientific Background Extracellular signal regulated kinase (ERK), also known as mitogen activated protein kinase (MAPK), is a family with two homologous members; ERK1 (MAPK3) and ERK2 (MAPK1). This antibody reacts with the active forms of these proteins that have specific phosphorylated Threonine and Tyrosine residues within a Thr-Glu-Tyr motif (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2).
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.