Left: Rat cerebellum stained for alpha II spectrin (green, 1:2,000) and GFAP (C-1373-50, red, 1:5,000). The spectrin antibody stains the submembraneous cytoskeleton on neurons and strongly reveals the cell bodies and dendrites of Purkinje cells, while the GFAP antibody stains the processes of Bergmann glia and astrocytes. Right: Western blot analysis of spectrin expression (green) in neural tissue and cell lysates.  protein standard,  rat whole brain,  rat spinal cord,  mouse whole brain,  mouse spinal cord,  NIH-3T3,  HEK293,  HeLa,  SH-SY5Y,  C6 glioma cells. A prominent band at about 250-260 kDa represents the intact alpha II spectrin heavy chain.