Product DescriptionMouse anti-Myosin-4 Monoclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Rabbit skeletal fast myosin (MYH4) has a predicted length of 1,938 residues and a MW of 223 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rabbit; rat;
Target Host SpeciesRabbit
Species ReactivityHuman, Mouse, Rabbit, Rat
Immunogen DescriptionRabbit muscle myosin
FormatLyophilized from mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS buffer to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage Instructions12 months after purchase at -20°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesMyosin-4; Myosin heavy chain 4; Myosin heavy chain 2b; MyHC-2b; MYH4;
Scientific BackgroundFast skeletal myosin belongs to the myosin heavy chain family. It is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits.