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Fibrillarin (Nop1p), Mouse Monoclonal Antibody

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Catalog Number

M-1372

Product Info

Product Name Fibrillarin (Nop1p), Mouse Monoclonal Antibody
Product Description google Mouse anti-rRNA 2'O-methyltransferase fibrillarin (Nop1p) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Alternative Names rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p
Application(s) ICC, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used as a marker for nucleoli in a wide variety of species. Human, Rat, Drosophila, S. pombe, C. elegans and and S. pombe. Predicted to react with other mammalian tissues.
Species Reactivity C. elegans, Drosophila, Human, Other Mammals (Predicted), Rat, Yeast
Immunogen Description Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
Conjugate Unconjugated
Purity Description Concentrated cell culture supernatant.
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-rRNA 2'O-methyltransferase fibrillarin (Nop1p) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Application(s) ICC, WB
Application Details Western Blotting (WB), Immunocytochemistry (ICC). A dilution of 1:2000 (cell lysates) to 1:10000 (nuclear fractions) is recommended for WB of yeast protein samples followed by chemiluminescent detection (ECL). For other non-ECL WB methods, a dilution of 1:1000 to 1:5000 is recommended. A dilution of 1:500 is recommended for ECL WB on mammalian fibrillarin. A dilution of 1:1000 to 1:5000 is recommended for IC on yeast cells and 1:500 for mammalian cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target rRNA 2'O-methyltransferase fibrillarin (Nop1p)
Specificity The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used as a marker for nucleoli in a wide variety of species. Human, Rat, Drosophila, S. pombe, C. elegans and and S. pombe. Predicted to react with other mammalian tissues.
Target Host Species Yeast
Species Reactivity C. elegans, Drosophila, Human, Other Mammals (Predicted), Rat, Yeast
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG
Clone Name 38F3
Conjugate Unconjugated
Immunogen Description Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
Purity Description Concentrated cell culture supernatant.
Format Lyophilized, without preservatives.
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 250 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p
Uniprot Number P15646
Uniprot Number/Name P15646 (FBRL_YEAST)
Scientific Background Fibrillarin is part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin such as this antibody can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A: Immunofluorescence staining of fibrillarin (Nop1p) expressed in nucleoli with M-1372-250 (green). Cells were double-immunolabelled with an antibody to neurofilament NF-H (red). Blue: cell nucleus, stained with DAPI.
B: Western blot of whole yeast protein extract with M-1372-250 (left lane). Right lane: no primary antibody control.
Left: Visualization of fibrillarin expression (green) in HeLa cells by Immunocytochemistry . Staining was performed with mouse anti-fibrillarin antibody diluted 1:10 from concentrated tissue culture media, and chicken antibody to vimentin (C-1409-50, 1:1,000, red). Blue: DAPI nuclear stain. The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments. Right: Western blot analysis of cell lysates with mouse antibody to fibrillarin (1:500, green). [1] protein standard, [2] C6 cytosol, [3] C6 nucleus, [4] HEK293 cytosol, [5] HEK293 nucleus, [6] NIH-3T3 cytosol and [7] NIH-3T3 nucleus. The band at 37 kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.

Citations & References

Specific References Paeschke K. et al (2005) Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo. Nat Struct Mol Biol. 2005 Oct;12(10):847-54.

Postberg J. et al (2005) Synthesis of pre-rRNA and mRNA is directed to a chromatin-poor compartment in the macronucleus of the spirotrichous ciliate Stylonychia lemnae. Chromosome Res. 2006;14(2):161-75.

Du T.G. et al (2008) Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA. EMBO Rep. 2008 Aug;9(8):781-7.

Srivastava L. et al (2010) Mammalian DEAD box protein Ddx51 acts in 3' end maturation of 28S rRNA by promoting the release of U8 snoRNA. Mol Cell Biol. 2010 Jun;30(12):2947-56.

Sasano Y. et al (2008) Distribution of U3 small nucleolar RNA and fibrillarin during early embryogenesis in Caenorhabditis elegans.Biochimie. 2008 Jun;90(6):898-907.

Meng L. et al (2007) Nucleolar trafficking of nucleostemin family proteins: common versus protein-specific mechanisms.Mol Cell Biol. 2007 Dec;27(24):8670-82.