Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Filensin (isoform 1) has a predicted length of 665 residues and MW of 75 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect Filensin in formalin/acetone fixed tissues.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesBeaded filament structural protein 1; Lens fiber cell beaded-filament structural protein CP 115; CP115; Lens intermediate filament-like heavy; LIFL-H; BFSP1;
Scientific BackgroundFilensin is a lens-specific intermediate filament-like protein found in the beaded filament of ocular lens. Defects in this gene are the cause of autosomal recessive cortical juvenile-onset cataract. At least 2 isoforms are produced by alternative splicing.