Application DetailsWB, ICC. Suggested dilution of at least 1:1,000 for ICC. Suggested dilution of 1:2,000 or lower for WB. Optimal concentrations/dilutions should be determined by the end-user.
TargetGalectin-3 (Gal-3)
SpecificityHuman; Mouse; Rat; predicted to react with other species due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Other Mammals (Predicted), Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG1
Clone Name5C21
ConjugateUnconjugated
Immunogen DescriptionFull length recombinant Galectin-3 expressed in and purified from E. coli.
Purity DescriptionAffinity purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundGalectin 3 is a lectin with carbohydrate recognition domains (CRD) which bind -galactoside. It is a multifunctional protein expressed both on the cell surface, cytoplasm and nucleus and appears to have roles in specific carbohydrate binding and in the regulation of mRNA splicing.
Image shows rat brain neural cultures stained with Mouse Monoclonal Antibody to Galectin-3 M-1574-100 (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.
Left: Analysis of galectin-3 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with mouse antibody to galectin-3 (red, 1:1,000), and co-stained with chicken antibody to MAP2 (C-1382-50, green, 1:10,000). Blue: Hoechst nuclear stain. The galectin-3 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while MAP2-antibody labels dendrites and perikarya of mature neurons. Right: Western blot analysis of tissue and cell lysates using mouse antibody to galectin-3 (green, 1:2,000): [1] protein standard, [2] mouse brain lysate; [3] - [7] rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at 30 kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with an antibody to HSP60 (red, lanes 2-8).
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