Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human GFAP has a predicted length of 432 residues and MW of 50 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect GFAP in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGlial fibrillary acidic protein (GFAP)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAstrocyte; Glial fibrillary acidic protein;
Scientific BackgroundGFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. At least 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons. This antibody detects all three isoforms.
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Staining of frozen sections from adult mouse spinal cord. Tissue was perfusion fixed and the primary antibody was incubated for 48 hours at 4°C, using antibody at a concentration of 1 µg/ ml. ALEXA-488 labelled goat anti-mouse was used as secondary antibody. Note the intense fluorescence associated with astrocytes.
General ReferencesReeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).