Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human GAP-43 has a predicted length of 238 residues and MW of 25 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect GAP-43 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGrowth-associated protein 43 (GAP-43)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; chicken; snake
Target Host SpeciesRat
Species ReactivityChicken, Human, Mouse, Rat, Snake
Immunogen DescriptionGAP-43 protein from neonatal rat forebrain membranes
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNeuromodulin; GAP43; PP46; p57; B-50; F1; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50;
Scientific BackgroundGAP-43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is also a major component of neuronal growth cones. GAP-43 is used to specifically label injured neurons and to score neuronal regeneration. This antibody reacts with the three known isoforms and to both kinase C phosphorylated and dephosphorylated forms of GAP-43.