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Growth associated protein 43 (GAP-43), Mouse Monoclonal Antibody

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Catalog Number

M-1650

Product Info

Product Name Growth associated protein 43 (GAP-43), Mouse Monoclonal Antibody
Product Description google Mouse anti-Growth associated protein 43 (GAP-43) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Alternative Names protein F1, neuromodulin, neural phosphoprotein B-50, axonal membrane protein GAP-43, calmodulin-binding protein P-57
Application(s) FC, ICC, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity The antibody reacts with a 43 kDa band by Western blot on rat spinal cord lysate. It has also been used successfully for immunocytochemistry.
Species Reactivity Mouse, Rat
Immunogen Description C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Conjugate Unconjugated
Purity Description Protein G purified immunoglobulin
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-Growth associated protein 43 (GAP-43) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Application(s) FC, ICC, WB
Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Flow Cytometry. A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC. Use 2ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Growth associated protein 43 (GAP-43)
Specificity The antibody reacts with a 43 kDa band by Western blot on rat spinal cord lysate. It has also been used successfully for immunocytochemistry.
Target Host Species Rat
Species Reactivity Mouse, Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG1
Clone Name GAP43
Conjugate Unconjugated
Immunogen Description C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Purity Description Protein G purified immunoglobulin
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names protein F1, neuromodulin, neural phosphoprotein B-50, axonal membrane protein GAP-43, calmodulin-binding protein P-57
Uniprot Number P06837
Uniprot Number/Name P06837 (NEUM_MOUSE)
Scientific Background GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A: Flow Cytometry analysis of GAP43 expressed in human neuroblastoma SH-SY5Y cell line. Fixing and permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS; Blocking: 1% BSA; Primary antibody: Mouse Monoclonal antibody to GAP43 (cat # M-1650-100, 2 μg per ~106 cells) for 30 minutes at room temperature; Secondary antibody: Goat anti-mouse PE (1:100 dilution), incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions. Data and results were generated using Orflo Moxiflow^TM instrument and protocols. B: Immunofluorescence analysis of mixed neuron-glial cultures stained with mouse anti-GAP43 (green) and rabbit anti-MAP2 (red). Blue: DNA staining. The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites. C: Western Blot analysis of GAP43 expression in whole rat spinal cord lysates. The antibody recognizes the ~43 kDa protein.

Citations & References

General References Skene JH, Willard M. Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells. J. Cell Biol. 89:86-95 (1981).
Wiederkehr A, Staple J, Caroni P. The Motility-Associated Proteins GAP-43, MARCKS, and CAP-23 Share Unique Targeting and Surface Activity-Inducing Properties. Exp. Cell Res. 236:103-116 (1997).