Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended for WB. Human HSP60 has a predicted length of 573 residues and MW of 61 kDa. A concentration of 4.0-8.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHeat shock protein 60 (HSP-60)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; chicken; rat;
Target Host SpeciesHuman
Species ReactivityChicken, Human, Rat
Immunogen DescriptionRecombinant human Heat Shock Protein 60
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesHeat shock protein 60; HSP-60; Hsp60; 60 kDa chaperonin; Chaperonin 60; CPN60; Mitochondrial matrix protein P1; P60 lymphocyte protein; HuCHA60; HSPD1; HSP60;