HeLa cells stained with M-1692-100 (red) and also with our chicken polyclonal antibody to Vimentin, (C-1409-50). The HSP27 antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins. The vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells.
Left: Analysis of HSP27 expression (green) in HeLa cells by Immunocytochemistry. HSP27 was stained with mouse antibody at 1:2,000 dilution, and cells were co-stained with a chicken antibody to HSP60 (red). Blue: DAPI nuclear stain. The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria. Right: Western blot analysis of HSP27 expression (green) in tissue or whole cell lysates using mouse anti-HSP27 antibody at 1:10,000 dilution.  protein standard,  rat brain,  mouse brain,  NIH-3T3,  HEK293,  HeLa. The strong single band at 27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific. The blot was simultaneously probed with a rabbit antibody to HSP60 (red, lanes 2-6).