Application DetailsThis antibody is recommended for WB, ELISA, sandwich ELISA, IHC and immunofluorescence. It is recommended to be used at 3 µg/mL for IHC and 10 µg/mL for immunofluorescence. This antibody has a detection limit of 0.03ng/mL in sandwich ELISA when using the recombinant protein. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetPAP-inositol 1,4-phosphatase (PIP)
SpecificityThe specificity of this antibody has been confirmed by WB against the recombinant protein and in HeLa cell lysate. This antibody has not yet been tested in other species.
Target Host SpeciesHuman
Immunogen DescriptionRecombinant protein of amino acids 1 to 100 of human PIP with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
PIP belongs to the inositol monophosphatase family. It hydrolyses adenosine 3'phosphate 5'-phosphosulfate to adenosine 5-phosphosulfate and 3'(2)'-phosphoadenosine 5'-phospate to AMP. It requires magnesium as a cofactor. Lithium is an uncompetive inhibitor and inositol 1,4-bisphosphate is a competitive inhibitor of PIP. Lithium is one of the drugs used to treat manic depression and the inhibition of PIP may account for lithium-induced nephrotoxicity.
Immunohistochemistry of mouse monoclonal antibody to human PIP [2E1], catalogue number M-805-100, at a concentration of 3 µg/mL, on formalin-fixed and paraffin-embedded human pancreas.
Immunofluorescence detection of PIP on HeLa cells when using mouse monoclonal antibody to human PIP [2E1], catalogue number M-805-100, at a concentration of 10 µg/mL.
Western blot analysis of PIP in HeLa cell lysate using the mouse monoclonal antibody to human PIP [2E1], catalogue number M-085-100. This anitbody recognises a band of approximate molecular weight 37 KDa