Product DescriptionMouse anti-Neurofilament heavy polypeptide (NF-H) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsThis antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNeurofilament heavy polypeptide (NF-H)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Immunogen DescriptionPartial recombinant human NF-H protein (263-364) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNeurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; NEFH; KIAA0845; NFH
Neurofilaments contain three intermediate filament proteins: L, M and H, which are involved in the maintenance of the neuronal caliber. NF-H has an important function in mature axons. NF-H is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Defects in NF-H are a cause of amyotrophic lateral sclerosis (ALS) which is a neurodegenerative disorder affecting upper and lower motor neurons and results in fatal paralysis.