Product DescriptionMouse anti-Variably charged protein X-A (VCX-A) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsThis antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetVariably charged protein X-A (VCX-A)
SpecificitySpecificity has been confirmed by WB and ELISA against the antigen. Human, mouse and rat. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionPartial recombinant human VCX-A (2-100) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesVariable charge X-linked protein 3; Variably charged protein X-A; VCX-8r; Variable charge protein on X with eight repeats; VCX3A; VCX3; VCX8R; VCXA
VCX-A is only expressed in testis and may play a role in spermatogenesis or in sex ratio distortion. It is a 186 amino acid peptide that has an estimated molecular weight of ~20 kDa. It was recently identified that VCX-A is an inhibitor of the decapping enzyme DCP2 and that it stabilised mRNA. It is a noncanonical cap-binding protein that binds to capped RNA but not cap structures lacking RNA. Defects in VCX-A are associated with some forms of X-linked nonspecific mental retardation.