Application DetailsThis antibody is recommended for WB, IHC and sandwich ELISA. For IHC, a concentration of 2 µg/mL is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetLymphocyte antigen 6H (Ly-6H)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name3E10
ConjugateUnconjugated
Immunogen DescriptionRecombinant human LY-6H protein with GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
The LY6 antigens are a family of glycosylphosphatidylinositol-anchored cell surface glycoproteins. LY-6H belongs to the LY6 family and it encodes a 140 amino acid polypeptide that is 23-33% identical to other known family members. LY-6H is higly expressed in the brain (cerebral cortex, amygdala, hippocampus and subthalamic nucleus) and in the acute leukemic cell line MOLt-3. It is also expressed at lower levels in testis, small intestine and colon.
Immunohistochemical detection of LY-6H on formalin fixed, paraffin-embedded, human tonsil tissue. Anti-LY-6H primary antibody, catalogue number M-845-100, was used at a concentration of 2 µg/mL.
Western blot detection of LY-6H in human chronic myelogenous leukemia cell lysate.