Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human p53 (isoform 1) has a predicted length of 393 residues and MW of 44 kDa. A concentration of 0.5-2.0 µg/mL is recommended to detect p53 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCellular tumor antigen p53
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Immunogen DescriptionRecombinant human wild-type p53 protein
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific Backgroundp53 is a DNA-binding protein containing transcription activation, DNA-binding and oligomerization domains. p53 is ubiquitously expressed and responds to a variety of cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair or changes in metabolism. Multiple p53 variants are produced from alternative promoters and alternative splicing.