Product DescriptionMouse anti-Autophagy-related protein 3 (ATG3) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsThis antibody is recommended for WB, sandwich ELISA and Flow Cytometry (2μg/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAutophagy-related protein 3 (ATG3)
SpecificitySpecificity has been confirmed by WB and ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Antibody IsotypeIgG2b, kappa
Immunogen DescriptionPartial recombinant human ATG3 [1-100] with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAutophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3; APG3; APG3L
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. ATG3 is an E2-like protein-conjugating enzyme for GABARAPL1, GABARAPL2 and MAPLC3 binding them to PE. ATG3 forms an E1-E2 complex with ATG7 and cross-talks with the ATG12 conjugation system. ATG3 is a cytoplasmic protein that is widely expressed with highest expression in heart, skeletal muscle, kidney , liver and placenta.
A: Flow Cytometry analysis of ATG3 expression in SH-SY5Y cells. Fixing and permeabilization of cells: absolute methanol and 0.1% Tween-20 in PBS; Primary antibody: Mouse Monoclonal antibody to ATG3 (cat # M-908-100, 2 μg per ~106 cells) for 30 minutes at room temperature; Secondary antibody: Goat anti-mouse PE (1:100 dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions. Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Western blot detection of ATG3 expression in Jurkat cell lysate. C: Sandwich ELISA using the anti-ATG3 monoclonal antibody as capture antibody. The detection limit for GST-tagged recombinant ATG3 is 1 ng/mL.